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Image Search Results
Journal: Biochemical and biophysical research communications
Article Title: Tribbles 3 Regulates Protein Turnover in Mouse Skeletal Muscle
doi: 10.1016/j.bbrc.2017.09.134
Figure Lengend Snippet: (A–B) Ten- to eleven-weekold female wild type (WT) and muscle-specific TRB3 transgenic (TG) mice were used to determine TRB3 mRNA and protein expression from tibialis anterior and gastrocnemius, respectively. (C) Body weight was similar between WT and TG. (D) Weights of tibialis anterior (TA), extensor digitorum longus (EDL), soleus (SOL), and gastrocnemius (GAS) muscles were measured. (E–F) Muscle function was evaluated by measuring grip strength and voluntary wheel exercise capacity for 8 weeks. (G) Frozen TA muscles were sectioned in 10-µm-thickness by a cryostat microtome and stained with hematoxylin and eosin. Original magnification: ×400; scale bar: 50 µm. (H) Fibers were traced and analyzed by ImageJ in order to determine the mean CSA. (I) The frequency distribution of the fibers in wild type and TRB3TG mice was determined. Data are the means ± S.E.M (n = 5–6 per group). * P < 0.05, ** P < 0.01 vs. WT
Article Snippet: We thank M. Montminy (Salk Institute) for providing the muscle-specific TRB3 transgenic mice, and
Techniques: Transgenic Assay, Expressing, Muscles, Staining
Journal: Biochemical and biophysical research communications
Article Title: Tribbles 3 Regulates Protein Turnover in Mouse Skeletal Muscle
doi: 10.1016/j.bbrc.2017.09.134
Figure Lengend Snippet: Ten- to eleven-week-old female wild type (WT) and whole-body TRB3 knockout (TRB3KO) mice were euthanized and GAS muscles were collected for analyses. (A–D) The levels of phosphorylation of signaling molecules, including mTOR (A), S6K (B), and Akt (C and D) were determined by Western blot. (E) Equally amount of protein lysates was analyzed for puromycin incorporation from SOL muscle. (F–G) The amount of puromycin incursion was determined in EDL (F) and SOL (G) muscles. (H–I) Muscle weights in TRB3KO mice were determined for TA (H) and GAS (I) muscles. Data are the means ± S.E.M. (n = 5–6 per group). * P < 0.05 vs. WT
Article Snippet: We thank M. Montminy (Salk Institute) for providing the muscle-specific TRB3 transgenic mice, and
Techniques: Knock-Out, Muscles, Phospho-proteomics, Western Blot